By E. E. Hansen, J. F. Hubstenberger, G. C. Phillips (auth.), Professor Dr. Y. P. S. Bajaj (eds.)
Twenty-seven chapters take care of the regeneration of vegetation from protoplasts and genetic transformation in quite a few species of Agrostis, Allium, Anthriscus, Asparagus, Avena, Boehmeria, Carthamus, Coffea, Funaria, Geranium, Ginkgo, Gladiolus, Helianthus, Hordeum, Lilium, Lithospermum, Mentha, Panax, Papaver, Passiflora, Petunia, Physocomitrella, Pinus, Poa, Populus, Rubus, Saintpaulia, and Swertia. those reports mirror the far-reaching implications of protoplast know-how in genetic engineering of crops.
This quantity is of specified curiosity to complicated scholars, lecturers, and examine scientists within the box of plant tissue tradition, molecular biology, genetic engineering, plant breeding, and common plant biotechnology.
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Additional resources for Plant Protoplasts and Genetic Engineering VII
Physiol Plant 15: 473--497 Orozco FJ, Schieder 0 (1984) Isolation of mesophyll protoplasts of the genus Coffea. Turrialba 34: 534-536 32 c. Schapke: Regeneration of Plants from Protoplasts of Coffea spp. (Coffee) Pfrunder R, Wanner H, Frischknecht PM, Baumann TW (1981) An attempt to localize caffein in the cell by its washout kinetics. In: Proc 9th Int Colloq Coffee, 16-20 June 1980, London. ASIC, Paris, pp 169-175 Reinert J (1959) Uber die Kontrolle der Morphogenese und die Induktion von Adventivembryonen an Gewebekulturen aus Karotten.
Dihaploid protoplasts of C. arabica coming from anther or microspore culture could be fused with diploid, somatic protoplasts of other coffee species to obtain tetraploid hybrids. Protoplasts might also be manipulated by uptake of organelles. An example would be the transfer of cytoplasmic male sterility from C. eugenoides to C. arabica. Somaclonal variation in plants regenerated from protoplasts could provide a new source of genetic variability in cultivated C. arabica, which has a very narrow genetic base (Sybenga 1960) and which is known to be quite resistant to radiation and to chemical mutagens (Sandahl et al.
The time required for plant regeneration lies in the range of 1 year, although Spiral and Petiard (1992) mention that they achieved regeneration within 6 months from protoplasts of C. arabica. Regeneration of Plants from Protoplasts of Coffea spp. (Coffee) 29 4 Summary and Conclusions During the past decade, substantial progress has been made in the isolation and regeneration of coffee protoplasts. Plant regeneration has been achieved for both of the major cultivated species C. arabica and C. canephora, as well as for the interspecific hybrid Arabusta.