By Y. H. Dan, C. T. Stephens (auth.), Professor Dr. Y. P. S. Bajaj (eds.)
In continuation of Volumes eight, nine, 22, and 23, this new quantity bargains with the regeneration of vegetation from remoted protoplasts and genetic transformation in a number of species of Actinidia, Allocasuarina, Anthurium, Antirrhinum, Asparagus, Beta, Brassica, Carica, Casuarina, Cyphomandra, Eucalyptus, Ipomoea, Larix, Limonium, Liriodendron, Malus, Musa, Physcomitrella, Physalis, Picea, Rosa, Tagetes, Triticum, and Ulmus.
These reviews mirror the far-reaching implications of protoplast know-how in genetic engineering of crops. The publication encompasses a wealth of precious details for complicated scholars, lecturers, and researchers within the box of plant tissue tradition, molecular biology, genetic engineering, plant breeding, and common biotechnology.
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Extra info for Plant Protoplasts and Genetic Engineering V
1993). For regeneration, the soft-type calli were plated, after 4 weeks in liquid K8p medium, onto solid PG o medium supplemented with 1 JlM BAP (Krens et al. 1990). After 2-3 weeks, individual calli were transferred to fresh plates of the same medium. Here, shoot primordia were generated within 1-3 weeks. ). This method has proved successful for a range of accessions/varieties (Table 3). For the best line, a 10-20% regeneration frequency could be obtained (Krens et al. 1990). Regenerated shoots were easily rooted on PG o medium supplemented with 25 JlM IBA.
Directly after isolation, protoplasts showed reduced DNA levels, although an increase could already be demonstrated after 1 day of culture. In seedlings the cotyledons and roots were predominantly 2C-4C and hypocotyls 16C-32C. After 4 years of culture, the suspension cultures in our laboratory initiated from callus formed on leaves proved to be more than 16C. This might be one of the reasons why, although yields and plating efficiencies are gene rally high, no plants have been regenerated from completely dedifferentiated sugar beet suspension cultures.
Schlangstedt et al. 5 M. , glucose. In our laboratory the basis of the isolation media is the CPW salt mix (Frearson et al. 1973) supplemented with 9% (w/v) mannitol ( = approx. 5 M). This concentration was chosen in preference to the originally used 13% (w/v) ( = approx. 7 M) mannitol, not because yields Were significantly enhanced, but because results could be better reproduced, protoplasts appeared healthier and, in culture, the best plating efficiencies were obtained only when protoplasts, isolated in 550 mOsmoljkg H 2 0, were cultured in medium with the same osmolality (Krens et al.