Plant Cells (Advances in Biochemical Engineering by Zhong, Jian-Jiang, volume editor

By Zhong, Jian-Jiang, volume editor

The new achievements in engineering reviews on plant phone cultures are reviewed, integrated are the fuel focus results and bioprocess integration for the improved productiveness of plant secondary metabolites. The metabolic engineering of plant secondary metabolite pathways and recombinant protein construction from genetically transformed plant cells are brought. Large-scale plant micropropagation through somatic embryogenesis and furry roots is mentioned for effective propagation of desease-free, genetically uniform and large quantities of vegetation in vitro in colossal quantities. Characterization and alertness of furry plant roots endowed with photosynthetic services can be coated during this distinct quantity.

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Extra info for Plant Cells (Advances in Biochemical Engineering Biotechnology, Vol. 72)

Example text

The four types of stoppered culture tests were compared using two-sample t tests on these quantities to determine whether the particular conditions affected the cell metabolism [74]. If either initial rate showed a statistically significant (95% confidence level) difference between the two test types being compared, then we concluded that the conditions affected metabolism and would be likely to affect bioreactor performance. Table 2 shows the comparisons made and the probability that the difference between the sets of data would occur if the sets were actually from the same population.

The productivity of respectively valuable metabolic compounds, generic phenolic compounds, artemisinin and paclitaxel, and aspects of culture physiology were also studied. Artemisinin is a promising antimalarial drug and paclitaxel is an effective anticancer drug, so progress towards improved methods of manufacturing would be very desirable. Moreover, N. tabacum, A. annua, T. cuspidata and T. canadensis can be considered as model systems; the methods (and some of the conclusions) developed from the current studies may be applicable to other phytoproduction systems [25–30].

Yunnanensis and T. hicksii. Because the evergreen Taxus brevifolia grows slowly (roughly a foot of height and a half inch of trunk diameter per decade), other techniques were considered to produce the compound without destroying T. brevifolia trees. Bristol-Meyer Squibb is currently manufacturing paclitaxel using a semi-synthesis from 10deacetylbaccatin III, which is isolated from needles of the Himalayan yew, T. wallinchina. Structure 2. Paclitaxel The cell culture process was licensed in May 1995 by Bristol-Meyer Squibb, which in 1998 designated $25 million for development of an FDA-approved commercial process.

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