Ciba Foundation Symposium - Growth Control in Cell Cultures by England) (1970 : Wolstenholme, G. E. W. ; Knight, Julie,

By England) (1970 : Wolstenholme, G. E. W. ; Knight, Julie, ; Ciba Foundation. Symposium on Growth Control in Cell Cultures London

Chapter 1 Chairman's advent (page 1): M. G. P. Stoker
Chapter 2 reviews of Serum elements Required via 3T3 and SV3T3 Cells (pages 3–15): Robert W. Holley and Josephine A. Kiernan
Chapter three stipulations Affecting the reaction of Cultured Cells to Serum (pages 17–32): G. D. Clarke and M. G. P. Stoker
Chapter four elements Controlling the Multiplication of Untransformed and reworked BHK2I Cells below a number of Environmental stipulations (pages 33–44): L. Montagnier
Chapter five the importance of floor constitution alterations for progress regulate less than Crowded stipulations (pages 45–69): Max M. Burger
Chapter 6 legislation of phone Multiplication in Tissue tradition (pages 71–87): Renato Dulbecco
Chapter 7 Molecular trade and progress keep watch over in Tissue tradition (pages 89–105): John D. Pitts
Chapter eight makes an attempt to Isolate SV40 Transformation issue (pages 107–125): R. R. Burk and Caroline A. Williams
Chapter nine progress legislation in Cultures of Chick Embryo Fibroblasts (pages 127–149): H. Rubin
Chapter 10 SV40 Transformation and mobile progress keep an eye on (pages 151–167): G. J. Todaro, C. D. Scher and H. S. Smith
Chapter eleven development keep an eye on among varied Cells in tradition (pages 169–186): R. A. Weiss and Dorothy L. Njeuma
Chapter 12 particular progress legislation in Early Subcultures of Human Diploid Fibroblasts (pages 187–206): Jonathan Bard and Tom Elsdale
Chapter thirteen shipping and Phospholipid adjustments with regards to keep watch over of Gsrowth in 3T3 Cells (pages 207–220): Dennis D. Cunningham and Arthur B. Pardee
Chapter 14 facets of Membrane constitution and serve as in Escherichia coli (pages 221–258): I. B. Holland, A. C. R. Samson, Eva M. Holland and B. W. Senior
Chapter 15 Chairman's last comments (pages 259–260): M. G. P. Stoker

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Some components require immunoelectrophoresis to distinguish them from gamma globulin and albumin, DISCUSSION 31 although they are not in fact gamma globulin and albumin. These proteins were shown to be also synthesized in uiuo, and are not turned on in response to cultivation in vitro. Finally, at least to some extent part ofthis sounds like the old conditioned medium story (Rubin 1966) ;it’s clear that cells make a factor in the presence of serum which enhances the growth of small numbers of cells.

The next question arises: what is the site of action of these effectors? (I) THE CELL SURFACE AS THE PRIMARY SITE OF ACTION OF MACROMOLECULAR EFFECTORS There are some indications that at least some of the effectors described act primarily at the cell surface. This particularly concerns the role of 38 L. MONTAGNIER sulphated polysaccharides and that of adenine nucleotides. The experimental evidence can be summarized as follows: (I) Role ofsulphated polysaccharides The inhibitory effect of SPS on cells at stage I is maximal when fractions of high molecular weight (dextran sulphate in the range of 5 x 105-2 x I O ~ ) are used.

In addition, the use of dialysed serum has enabled us to detect a new amino acid requirement for the growth in all conditions of cells at stage zb: that for L-serine (Montagnier 1970~). M U L T I P L I C A T I O N OP BHK2I CELLS FIG. I. A diagram of the multi-step transformation of BHKzr cells. The conclusions to be drawn from these studies are: Transformation of BHK cells is not an all-or-none phenomenon; one can speak in this case of a multi-sequential transformation,comparable to the progression of transplanted tumours in vivo.

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