By Michael T. Ru, Jonathan S. Dordick, Jeffrey A. Reimer, Douglas S. Clark (auth.), Evgeny N. Vulfson, Peter J. Halling, Herbert L. Holland (eds.)
In contemporary years, enzymatic catalysis in natural solvents-as against aqueous solutions-has received significant recognition as a strong new method of the guidance of usual items, prescribed drugs, high quality chemical compounds, and meals parts. In Enzymes in Nonaqueous Solvents: tools and Protocols, top chemists, biochemists, biotechnologists, and method engineers summarize for the 1st time a variety of tools for executing enzymatic alterations less than nonaqueous stipulations. each one procedure comprises precise step by step directions for its winning crowning glory, an inventory of fabrics, and ancillary notes that specify the clinical foundation of the method, in addition to troubleshooting. additionally supplied are a popular description of key reactions, recommendation on biocatalyst education, dialogue of response stipulations, and directions on bioreactor layout.
accomplished and cutting-edge, Enzymes in Nonaqueous Solvents: tools and Protocols deals latest artificial chemists, biochemists, and procedure engineers all of the crucial details had to perform enzymatic reactions in nonaqueous media, in addition to to effectively scale as much as construction quantities.
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Extra info for Enzymes in Nonaqueous Solvents: Methods and Protocols
The gels thus formed by photo reaction have a higher mechanical strength than those prepared by thermochemical reaction. Urethane resin prepolymers with different properties (chain length and/or hydrophilicity–hydrophobicity balance) can form gels by reacting with water. The prepolymers have functional isocyanate groups in the molecules, which can polymerize through urea linkages in the presence of water (2–5). The features of the prepolymer methods are summarized as follows: 1. The entrapment procedures are simple and mild.
S. (1991) An introduction to industrial biocatalysis, in Biocatalysts for Industry (Dordick, J. ), Plenum, New York. 5. Khmelnitsky, Y. L. and Rich, J. O. (1999) Biocatalysis in nonaqueous solvents. Curr. Opin. Chem. Biol. 3, 47–52. 6. Uhlig, H. (1998) Application of technical enzyme preparations, in Industrial Enzymes and Their Applications (translated and updated by Linsmaier-Bednar, E. ), Wiley, New York. Imprinting Enzymes for Use in Organic Media 17 7. Dabulis, K. and Klibanov, A. M. (1992) Molecular imprinting of proteins and other macromolecules resulting in new adsorbents.
2. Gently mix the solution for 15 s. 3. Flash-freeze the enzyme–substrate solution in liquid N2. 4. Lyophilize the mixture for 24 h (see Note 2). 5. Store the imprinted enzyme in a desiccated environment at –20°C. 6. If necessary, the imprinting compound may be removed from the solid enzyme preparation by washing with a suitable anhydrous organic solvent (see Note 3). 2. Measurement of Enzyme Activity 1. Suspend 1 mg of the imprinted enzyme in 1 mL of anhydrous solvent containing nucleophile and acyl donor in a screw-cap vial.