By Michael Lynch
Michael Lynch's doctoral thesis and one of many first 3 laboratory stories along Bruno Latour and Steve Woolgar's "Laboratory Life" and Karin Knorr-Cetina's "The Manufacture of Knowledge".
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Extra resources for Art and Artifact in Laboratory Science: A Study of Shop Work and Shop Talk in a Research Laboratory (Studies in Ethnomethodology)
The analytic marking of instances of intact and degenerating terminals provided a basis for computing ratios of intact/degenerating terminals for each five micron square sector. When these ratios were plotted in terms of a series of "days" post the lesion, they were consulted to show the sequential changes in terminal density and the distribution which were used to document axon sprouting. 45 ETHNOGRAPHIC ACCOUNTS OF SHOP WORK THE LAB SETTING Key a= b= ''mß. ^ A ^ \s Ä r ••A . Ö- - 7- lÄlliÄ astrocyte protoplasm border between adjacent micrographs in the montage.
At room temperature, the brain is removed and placed in Solution C, and small pieces of tissue carefully sliced. The tissue is postfixed with buffered 1% osmium tetroxide for 3 hours. M. room 2 (one of the rooms used by the electron microscopists), cage with 5 rats with stitches in heads (from lesion). Rats have numbers written twice on tails. M. project) Rt. Ent. m. (date of lesion) #1, 2, 3 (number for rats in that cage) Other tag: Gold B. January 26, 1975 R. March 28, 1975 (biographical data for rats) Next to cage - seven syringes, a metal box with wood shavings inside, sink, rubber pad, hoses with clamps leading from inverted bottle labeled "conc," collection of scissors, clamps over to the side, manual open to section on perfusion.
A tour of the lab environment revealed, at any given time, a wide variety of tasks performed by different practitioners in conjunction with different instrumental apparatuses. " These simultaneously 55 ETHNOGRAPHIC ACCOUNTS OF SHOP WORK performed activities showed little ostensible relation to one another, at least to my relatively naive observation early in my inquiry. In later interactions with lab members, I learned that no single member had detailed access to the total range of activities in the overall research program.